FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329270
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15150.041.3691021
TCTGTCG13750.040.843228
CTGTCGC17600.033.5451139
TCGCTCT16400.032.4869844
TCTCGCT19900.030.0348072
GTCTTGC28950.029.3631671
CGCTCTG18150.027.7678935
CTCGCTC19500.027.6953543
GTATTAG13450.027.3160151
GTCTTAC12400.026.7242551
GTGTTAG22650.026.3985061
GGGTTTC52850.026.3076341
GGTATAC6300.025.1565781
GTTTCAC50000.024.482693
GTCTCAC36350.023.9800661
GTGTAAC10050.023.6546941
GTATTAT10400.023.551311
GTTAGCC19000.023.118333
GTATAGG8500.022.883071
GGAGTGC75000.022.8474245
Produced by FastQC (version 0.11.5)