FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955592
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15850.044.51688
GTCTCGC16000.040.4954071
CTGTCGC20650.034.8663989
TCGCTCT18100.032.618384
GTCTTGC34150.030.7783531
TCTCGCT19250.030.6666532
CTCGCTC22000.028.4723783
GTGTTAG26550.026.8444141
CGCTCTG21800.026.0914215
GGTATAC8250.025.3062211
GTGTACG5900.024.404011
GTATTAT13650.024.2609841
TTACACT20200.023.1680344
TACGCTT2502.423756E-623.0397195
GGGTTTC62000.022.9909421
GTTTCAC54350.022.5204223
GTCTCAC47450.022.4547791
TTGCTCT47900.022.2460754
GTCTTAC16200.022.21971
GTGTTAC15000.022.0774971
Produced by FastQC (version 0.11.5)