FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430119
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.043.788778
GTCTCGC10950.037.919861
TCGCTCT11500.037.563214
CTGTCGC13150.032.847939
TCTCGCT13650.030.0643272
CTCGCTC13650.028.4819933
GTATTAC5900.028.397611
GTCTTGC22650.028.3022141
GTATAAC4400.028.1450611
CGCTCTG14050.027.6694135
GTCTCAC27900.027.4151761
GTGTTAC10250.027.0063361
GTATAAT8700.026.7939451
GTGTTAG16550.025.5291271
GTATTAG9050.024.9527871
GTCCTAC7750.024.4386711
GTATTAT8050.024.4328331
GTACGGG1801.4727985E-424.2820151
GGTATAC4700.023.2487351
GTCTAGC10100.023.0799331
Produced by FastQC (version 0.11.5)