FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696393
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13500.042.6616868
GTCTCGC16400.035.0736121
CTGTCGC17950.033.2885939
GTCTTGC32200.032.5614551
TCGCTCT18250.032.353034
TCTCGCT19800.027.6383722
CGCTCTG19650.026.7449685
CTCGCTC20000.025.921883
GTACGGC2258.784409E-725.8883691
GTCTCAC40500.025.5288091
GTCTTAC13250.024.7282751
GTGTAGA19200.023.891121
GTGTAGC14900.023.4559041
GTGTAAC11250.023.2995321
GTATACG3452.8285285E-923.215111
GGGTTTC61650.023.1483551
GTGTTAG25150.022.871061
TTGCTCT42300.022.639944
CTTGCTC43200.022.1682763
GTTTCAC54900.022.1655453
Produced by FastQC (version 0.11.5)