FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815238
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15750.039.770398
GTCTCGC16850.038.7336541
CTGTCGC19700.033.258459
GTCTTGC34100.031.8994161
TCTCGCT19700.031.8036272
TCGCTCT18700.029.2628024
CTCGCTC19600.027.184393
GTATAAC7800.026.961821
CGCTCTG21600.025.6663325
GTCTTAC15000.024.656121
GTATTAC10150.024.2917461
GTATTAG13550.024.0834711
AACCGCG2502.424009E-623.03947
GTATAGG11100.022.8660371
TTGCTCT49400.022.4459344
GTGTTAG26700.021.9998441
GTGTACG4659.094947E-1221.8333781
GTACTAG7950.020.9800511
CTTGCTC47600.020.8744983
GTGTAAC11250.020.6273421
Produced by FastQC (version 0.11.5)