FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815238
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14750.040.9958468
GTCTCGC15650.033.599131
GTCTTGC33650.031.2526821
CTGTCGC18900.028.948579
TCGCTCT16350.028.6182144
TACCCCG2955.2568794E-1026.8421675
GCGTAGT4000.025.9210191
GTCTAAG9950.025.306951
CTCGCTC19400.024.4890563
GTACTAG6700.024.3182241
GTGTAAT16100.023.9200151
GTGTAAC12850.023.6300541
TACTCCG3702.8558134E-1023.346755
GTGTTAG24400.023.0678861
GTATTAT12700.022.7428471
GTATTAC8800.022.722971
GTGTTAC15750.022.570681
CGCTCTG21100.022.1757245
TTGCTCT44750.022.0381154
TCTCGCT21700.021.9550172
Produced by FastQC (version 0.11.5)