FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697590
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14900.043.0021488
CTGTCGC18950.036.4710669
GTCTCGC15800.035.9528051
TCGCTCT17250.033.811054
GTCTTGC32300.032.2425041
TCTCGCT19200.031.127172
TCGTCGC1050.00798005627.4256619
CTCGCTC20950.027.1522483
GTATTGG13100.026.6848771
TAATACT10450.026.1836434
CGCTCTG20150.026.0815985
GTATAGT9950.024.8857691
GTGTAGA19800.023.1723771
TTGCTCT46150.022.6234634
GTATAAG11300.022.5571821
GTATTAC9000.021.8482441
GGGTAAG18050.021.787721
GTCTAGC16100.021.712541
GTGTTAG22950.021.578511
GGAGTGC88950.021.5289125
Produced by FastQC (version 0.11.5)