FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697590
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15750.039.1862751
TCTGTCG14550.036.622528
TCGCTCT17300.035.3714184
CTCGCTC19050.030.6104453
GTATTAG10200.029.8981781
GTCTTGC33300.029.6544781
TCTCGCT20150.027.8675772
CTGTCGC20400.026.8271589
GTATAAG11650.026.8002071
GGTATAC6900.026.307921
GTGTTAG23000.025.57131
GTATTAA9450.024.5874651
GTGTTAT14600.024.3690621
CGCTCTG21500.024.1086945
TACACCG3402.7230271E-923.2912835
GTCTCAC41400.023.1509721
GAGTGCA88250.023.0045666
GTTTCAC52500.022.4886913
ATACCGT2603.4101868E-622.151156
GTACGAC1650.002579394322.0029891
Produced by FastQC (version 0.11.5)