FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497747
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.045.528948
GTCTCGC14400.041.454471
CTGTCGC16000.036.896229
TCGCTCT16850.035.0399974
TCTCGCT17500.032.9156232
GTCTTGC32800.031.0723571
TAGTCCG1555.774053E-527.8681145
CTCGCTC19650.027.848463
GTGTTAG22000.027.4647371
CGCTCTG19650.026.378985
GTCTCAC39950.025.5112251
TAGACCG2051.3636896E-524.5828485
GTATTAG11350.024.372931
GTTAGCC17750.024.3390183
GTATAGG9900.023.5306991
GTGTTAC14000.023.399391
GGAGTGC80800.023.3441455
GTGTTAT13600.023.0170461
GAGTGCA87100.022.978136
TCTGTCA44450.022.8368078
Produced by FastQC (version 0.11.5)