FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497747
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14800.041.8430028
GTCTCGC14250.038.2015081
GTCTTGC29900.032.7715261
TCGCTCT16450.031.5099094
CTGTCGC20100.030.809779
GTCTTAC14900.027.2794651
GTCTCAC41150.026.6342961
TCTCGCT19800.026.5426582
CGCTCTG19450.025.9094985
CTCGCTC20100.025.4301553
TAATACT9800.024.9766034
GTGTAGG21300.023.512761
GAGTGCA83900.023.3392816
GGAGTGC77750.023.240955
GTATAGG8750.023.2265171
GGGTTTC58000.023.1514321
GTGTACG4750.022.9209061
GTGTAAG16550.022.3669241
GGTATAC7200.022.1780991
GTATAAT10600.021.9118081
Produced by FastQC (version 0.11.5)