FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701643
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14500.038.5455321
TCTGTCG16000.035.5506448
TCGCTCT18400.030.9115184
CTGTCGC21650.028.9335489
TCTCGCT19500.028.4297472
CTCGCTC20250.027.0212523
GTCTTGC33350.026.9884011
CGCTCTG19600.026.447675
GTGTTAC16300.023.6014981
GTATTGG11500.022.7224941
GTCTCAC44000.022.600561
GTATAGG9350.022.5132241
GTATTAC7800.022.3340721
GTATTAG11400.022.2850951
GTATTAA11400.022.2850951
GTATTAT12500.022.0660651
TACACTG21450.021.8171025
GTGTAAA24300.021.8055931
GCGTAGT3355.4404154E-821.6673851
GTGTAAC11750.021.6212841
Produced by FastQC (version 0.11.5)