FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525903
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13400.044.592328
GTATTAC9550.034.3286441
GTCTCGC14600.032.4345551
GTCTTGC32350.031.0779441
CTGTCGC18000.030.7967579
TCGCTCT15250.027.3834424
TAATACT9750.027.3229124
GTATTAG11700.026.7750361
TTCACCG9950.026.771055
GTGTAAT14000.025.498551
TCTCGCT17100.025.2630082
GTCTCAC41650.025.1880741
GTCTTAC13200.024.8362521
GCGTAAC1500.001453319724.2843341
GTGTATA17800.024.1479051
GTACTAG7000.023.9374161
TCACCGT12650.023.3335116
CTCGCTC17500.023.0398623
GTTTCAC53500.023.0129453
GTGTAAG16500.022.9597361
Produced by FastQC (version 0.11.5)