FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918230
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15450.037.746888
GTCTCGC17650.034.0742871
CTGTCGC18300.031.4756349
TCGCTCT18400.029.74014
CTCGCTC21000.028.1152023
GTCTTGC34500.027.5134851
TCTCGCT21450.026.8574492
CGCTCTG20150.026.7988555
GTATAAC7050.024.6669541
GTCTCAC43350.022.899441
GTGTTAG28500.021.8648741
GTGTAAA23200.021.8626841
GTGTCGC3706.708433E-921.5419181
GTGTAGG22350.021.397341
GTATAGA12350.021.1217041
GTACTAT10750.020.8952051
GGAGTGC83950.020.8408575
GTCTTAC15250.020.4311121
TCGATCT14550.020.2888059
GTATAAG12150.020.2766421
Produced by FastQC (version 0.11.5)