FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775851
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15350.042.2127238
GTCTCGC17150.038.4520531
CTGTCGC18100.035.402369
TCGCTCT18800.032.936594
GTCTTGC36100.032.3192251
TCTCGCT21100.031.7400442
CTCGCTC20350.030.4279043
GGTATAC8300.028.8123131
GTATTAC8350.027.771911
CGCTCTG22050.026.7740765
GTGTTAG26450.026.0279641
GTATAAA12700.025.106791
GTGTTAC18250.023.8248751
GTCTTAC14800.023.0132751
TTGCTCT48550.022.9869044
GTATTGC10750.022.91991
TCTCGAT13200.022.9078647
GTATAGA11450.022.7844891
TCGATCT14750.022.453639
GGGTTTC60750.022.4261041
Produced by FastQC (version 0.11.5)