FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775851
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15900.039.388828
GTCTCGC17400.039.080831
GTCTTGC36150.033.3276331
CTGTCGC21050.032.8303689
TCGCTCT18300.031.862444
CTCGCTC21150.028.9299643
CGCTCTG21000.028.4514165
TCTCGCT22500.026.94562
GTGTAAG20050.026.1739431
GGTATAC7850.025.4225621
GTCTTAC15150.025.3697341
TTGCTCT47800.024.5473614
GCGTTAC2751.6360718E-724.1899531
GTATTAG11650.024.1092031
GTATAGC8800.023.5180071
GTCTCAC42750.023.341181
GTGTAAA24100.022.6955051
GTGTTAC17300.022.6440931
CTTGCTC47700.022.3348313
TCTGTTG40400.021.9166728
Produced by FastQC (version 0.11.5)