FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573921
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15600.033.6853378
GTCTCGC15850.031.2581921
GTCTTGC35450.028.3688011
CTGTCGC20500.025.9852319
TCGCTCT16550.025.6623654
GTGTTAG22500.025.6348271
GTATAAC6500.025.0280861
GTATAAT11050.023.4220051
GTATTAG13000.023.3216251
GTATTAC9700.022.8700891
GTCTCAC41100.022.8496861
GTATAGC8500.022.6189651
GTGTAAG20050.021.7598531
GTACTAC6850.021.5902541
TCTCGCT19550.021.409512
TTGCTCT48850.021.3671254
CTCGCTC18650.021.2291493
GCGTAAC2103.778105E-421.1276051
GTATAAG11100.020.6517581
TAATACT11300.020.3851624
Produced by FastQC (version 0.11.5)