FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716659
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15300.037.6473468
GTCTCGC16900.034.3130041
TCGCTCT17700.032.544814
GTCTTGC35900.030.0848081
CTGTCGC19400.029.6913479
GGTATAC8900.026.8769111
TCTCGCT20500.026.3476892
CTCGCTC20900.026.1837793
CGCTCTG20250.025.956455
GTATTAA11800.024.57161
GTACGGA3352.1191227E-923.8014451
GTATAAT13200.023.0637971
GTATAGG10100.022.965931
GTGTATA21950.022.7860981
GTGTTAC17000.022.1722551
GTATAGA12100.022.1652071
CGGTACT2603.404386E-622.1555024
TTGCTCT50400.022.0016464
GGGTTTC64650.021.8635921
GTGTAAA22700.021.7139321
Produced by FastQC (version 0.11.5)