FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716659
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15250.035.8728141
TCTGTCG14400.035.4943588
GTCTTGC34400.032.6654551
TCGCTCT17200.029.716214
CGCTCTG18650.027.4054645
CTGTCGC18400.026.9975269
GTGTAAC12300.026.445461
CTCGCTC21200.025.4662843
GTACTAT8750.025.3464281
TCTCGCT21800.025.155822
GTATTAG12650.023.3761521
GTATTAC9150.022.6224941
GTGTAAT15200.022.3726671
GTATAAC7650.022.2264421
GGTATAC8350.022.1338561
GGCTAGA21250.021.9172041
GAGTGCA91400.021.8168096
GTATAAG11300.021.5893231
GGAGTGC82300.021.2551465
CTTGCTC46450.020.9212783
Produced by FastQC (version 0.11.5)