FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374594
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.044.261598
GTCTCGC14750.041.1600881
TCGCTCT16950.036.5309334
CTGTCGC16250.032.788149
CGCTCTG18000.031.5994935
TCTCGCT19000.031.4614542
CTCGCTC19850.031.193923
GTCTTGC30700.029.8988441
GTATACG2207.7007644E-726.2818741
GTATTAG12200.026.066451
GTGTTAG22200.025.3939741
GTCTTAC12800.024.8445851
GTATTAT11250.023.7704961
GTGTAGG16750.023.732141
GTCCTAC10950.022.44161
TTACACT16200.022.2221974
GTCTCAC38800.022.1668661
GTACGGG2953.9978295E-722.0500471
TTGCTCT44500.022.0044694
GTATTGA10350.021.647631
Produced by FastQC (version 0.11.5)