FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786230
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14850.046.0595058
CTGTCGC18450.039.0244879
GTCTCGC15050.038.0215841
GTCTTGC39300.036.3089181
TCGCTCT18600.030.1944184
GTCTCAC42850.028.0605951
TCTCGCT18800.027.9660112
GTCCTAC11700.025.382691
GTATAGG10750.024.930611
TTGCTCT50050.024.8878334
TAATACT10800.024.6673774
CGCTCTG21450.024.5032335
GTGTAAC12550.024.2407021
GTGTTAC15950.024.068821
GTCCGGA4550.023.8791851
GGTATAC7300.023.8137631
CTCGCTC21750.022.842043
TTCACCG10850.022.561975
GGGTTTC61350.022.550621
TCACTCT52300.022.0274054
Produced by FastQC (version 0.11.5)