FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803771
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15200.039.7883838
GTCTCGC17000.039.2048341
TCGCTCT18150.036.1000864
CTGTCGC17000.034.728929
TCTCGCT21550.029.4067752
GTCTTGC36050.028.9372941
CTCGCTC23200.028.5524463
CGCTCTG22500.028.4795975
GTCTCAC44100.025.7906191
GTCTTAC14900.025.7685681
GTATTAC9200.024.4103811
GTGTTAC16850.024.0762481
GCGTTAT2451.9274012E-623.655091
CTGTTGC38500.023.1892839
GTCGTAA1902.2063097E-422.876961
GTATTAT12450.022.6932131
TAATACT10500.022.6291434
GTGTAAA23200.021.8580171
GGTATAC8750.021.5261331
GGAGTGC86650.021.4376665
Produced by FastQC (version 0.11.5)