FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803771
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16550.040.45188
GTCTCGC15700.034.33361
CTGTCGC21550.033.4054269
GTCTTGC33900.032.4550821
GTATTAA10350.027.8240531
TCGCTCT18050.027.1196824
GTATTAG13650.026.5069391
GTCTTAC14050.026.2778471
GTGTAAC11600.026.0988881
GTATAAT11550.024.9332431
TACACTG23400.024.6105485
GTGTAAA24450.024.4625841
TTACACT19750.024.0563494
TCTCGCT21150.023.8875922
CGCTCTG19250.023.5590085
GGGTTTC59900.023.1753961
CTTGCTC44750.022.6815763
GTCTCAC43800.022.590551
GTTTCAC56750.022.0714343
GAGTGCA92250.022.0054326
Produced by FastQC (version 0.11.5)