FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3812561
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14850.034.907728
GTCTCGC15300.031.2560081
GTCTTGC36300.028.942921
CTGTCGC18950.028.875239
TCGCTCT17950.028.4798564
GTATTAG14100.025.694011
GTCTTAC14700.024.6452751
GTGTTAC14500.024.4855041
TCTCGCT19800.024.3693892
TCTATCG1801.5949215E-423.9990568
GGTATAC7950.022.7852541
GTGTTAG25950.022.616671
CGCTCTG20300.021.989575
GTACTAC6600.021.95671
GTGTTAA17500.021.9441531
GTATTAC10600.021.8738461
GGGTTTC65900.021.7701191
GTTTCAC59900.021.6366273
CTCGCTC20700.021.5657833
CTCGATC15850.021.3493188
Produced by FastQC (version 0.11.5)