FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3812561
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16600.037.7294778
GTCTTGC37800.033.4377021
GTCTCGC16250.030.4756851
CTGTCGC21150.030.2942839
GTGTAAC12050.027.6030791
TCGCTCT17650.026.5113964
GGTATAC7600.025.286681
TTGCTCT49950.024.2123934
GTCTCAC42450.024.2029971
GTATTAT11450.022.5940781
GTGTTAG26150.022.3299351
GTATTAC9600.022.328461
CTCGCTC19900.022.0659983
CTTGCTC50000.022.0276553
TACACTG23700.021.8696775
GTACTAA9950.021.5430371
GTGTAAT14950.021.2598081
TAATACT11950.021.0845384
GTACGGA3909.784344E-920.8477951
GTGTAAG19550.020.7944761
Produced by FastQC (version 0.11.5)