FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333666
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10250.038.229811
TCTGTCG8850.036.6077468
TCGCTCT9950.036.1777344
GTCTTGC20650.033.38381
TCCGTCG900.003755520631.9978798
GTCTTAC8300.031.4743421
TCTCGCT11650.030.2806072
TTCACCG6600.029.4519635
CTGTCGC11500.029.4241399
CTCGCTC12200.027.1451633
GTATTAG7400.026.4767281
CGCTCTG12300.025.7538415
GTGTTAG16800.024.6205541
GTCTCAC27050.023.3391081
GTATAGG4700.023.1593131
TCACCGT8750.023.0384756
GTGTAAC8250.022.8692361
GTGTTAA10550.022.6983221
GTCTCGA20950.022.6810766
GAGTGCA60600.022.216356
Produced by FastQC (version 0.11.5)