FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333666
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9300.033.87121
GTCTTGC20150.033.4470521
TCTGTCG10100.032.073918
TCGCTCT10850.029.1933354
GTATTAA6450.028.3939231
GTATAAC4150.028.2433991
GTGTTAG15750.027.4420471
GTGTAAC7750.027.4120431
GTATTAT6700.027.3344461
GGTATAC4650.026.7818811
GTATTAC5500.026.6386621
TAATACT5900.025.6228854
CTGTCGC13600.025.407579
TATACTG6700.024.7123365
CGCTCTG12550.024.6652475
GTGTACG3301.5752448E-924.4187721
GTATTAG7250.024.2503681
ATACTGA9000.023.9960376
GTACTAT4900.023.9204311
CTAGAGT11500.023.7873743
Produced by FastQC (version 0.11.5)