FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820353
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11550.043.3484571
TCTGTCG11400.036.63068
TCGCTCT13250.034.776434
GTCTTGC24300.032.2495771
TCTCGCT14400.031.9991462
CTGTCGC13700.031.5320039
CGCTCTG13850.029.6309785
CTCGCTC14300.029.2020173
TAGCGCT2354.6036803E-827.573734
GTATTAG9950.024.79491
GTATAAT8000.023.5825021
GTACTGA9050.023.2517661
GTCTCAC31050.022.6681791
GTTTCAC39850.022.4034163
GTGTAAC8800.022.2632031
TTCACCG8450.022.1532555
GTCTTAC9500.022.1503661
GTGTAGA12800.022.1085971
ACCGTGT9250.021.7940128
GTGTACA15450.021.6040861
Produced by FastQC (version 0.11.5)