FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820353
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10400.042.2245948
TCGCTCT11600.038.4764484
GTCTCGC12400.036.6054081
CTCGCTC13450.032.1136933
GTCTTGC22000.030.6154331
TCTCGCT13750.030.3670062
GTATTAG9150.029.6043761
GTCTTAC9150.029.6043761
CTGTCGC15650.028.0597939
CGCTCTG13650.027.951465
GTATTAT7400.026.7120531
GTATAAA11350.026.4461981
GTACTAG4800.025.9288311
GTATAAG7200.024.4036061
GTCTCAC31900.023.4091031
TACACTG15450.023.2971485
GTATTGC8300.022.9335081
GTACAAG9350.022.70711
GTCTAGC10600.022.101381
GTATTAA8950.022.0859471
Produced by FastQC (version 0.11.5)