FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749921
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.044.365028
GTCTTGC25150.035.4945951
CTGTCGC13400.034.3891339
GTCTCGC11500.030.9238341
TCGCTCT12150.030.8152164
GTATTAA7550.028.8382821
GTATTAG8850.027.0623651
GTCTTAC10650.026.5772511
GTATAGG8300.026.2324121
GTCTCAC30450.025.979711
GTGTTAG18400.025.6383821
GTCCTAA9950.025.5293661
TTGCTCT31400.025.452564
GGGTTTC42800.025.4356331
CTTGTCG2551.0153963E-725.412569
GTGTAAC8950.025.1381741
GTCCTAC7900.024.8045731
CTTGCTC29850.024.6033343
TCTCGCT14150.024.4243452
GTATAAT7350.023.6983991
Produced by FastQC (version 0.11.5)