FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749921
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.036.9862258
GCGTACG1208.9057285E-636.6322821
GTCTCGC10450.036.457011
GTATTAC5600.032.7073941
GTCTTGC23500.030.2411171
GTGTTAG18000.029.3058241
TCGCTCT11650.029.0435984
CTGTCGC13500.028.2631519
TCTCGCT13550.028.1593692
GTATTAG9050.026.7152561
GTGTAAA13850.026.4492991
GTATAAC5550.026.4016461
CTCGCTC13400.026.3250853
GTCTTAC9050.025.9057031
CGCTCTG13900.024.8602185
GTGTAAC7750.024.5790791
GTCCGGT1902.0440023E-423.1361791
GTATAGG6650.023.1361771
GTATTAT7700.022.8357091
TTAGACT9000.022.3972154
Produced by FastQC (version 0.11.5)