FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793319
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17900.039.4179158
GTCTCGC16900.033.660321
TCGCTCT18850.031.7033044
GTCTTGC35150.029.704381
CTGTCGC24850.028.9730519
GTATTAG12450.027.7619361
GTATTAA11650.027.1959741
TCTCGCT20350.026.535952
CTCGCTC20700.025.3916133
CGCTCTG22450.025.335615
TTATACT9750.024.3695284
TAATACT10650.024.3383264
GTGTTAG24750.023.5660951
GGTATAC7950.023.549631
GTAATAC9550.023.3720133
GTCTTAC13400.023.1068921
GTTAGCC20150.022.511443
GTATAAC6450.022.3279131
GTCTCAC43950.021.954511
GTGTAAT16850.021.7945611
Produced by FastQC (version 0.11.5)