FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686485
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12300.043.0817831
TCTGTCG10100.040.6355938
GTCTTGC24100.033.13231
CTGTCGC13250.032.06199
TCGCTCT12050.031.6666094
GTATTAT9450.031.4940071
TCTCGCT14350.031.10662
GTCTTAC10350.027.3526971
TACCGTA1907.5550743E-626.5270847
CTCGCTC14900.026.0927733
GTACTAT6750.024.7343181
GTATTAC6300.024.1966171
TTGCTCT33750.023.46564
GTATAGT7600.022.9231131
GTATAGA8250.022.87681
GTATAAA11750.022.858081
GTGTAGT15900.022.8269961
GTAATAC5700.022.7358073
GTATTAA8000.022.6843281
CGCTCTG14000.022.6275425
Produced by FastQC (version 0.11.5)