FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717637
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12000.048.985881
TCTGTCG11000.041.8910038
TCGCTCT12700.040.24914
TCTCGCT13750.037.699132
CTGTCGC14850.033.4546249
CTCGCTC14650.032.925983
GTCTTGC24550.032.2212331
CGCTCTG14900.029.9575965
GTGTTAC10700.027.807841
GTCTCAC29050.025.9809111
GTCTTAC10450.025.6952341
GTGTATA12550.025.4434591
GTATAAT8400.024.190561
TTGCTCT32250.023.216954
GTGTTAG17700.022.9605311
TCTGTCA33000.022.6909628
GTATTAA8050.022.5377881
TAACACT13850.022.3521984
GTTTCAC37700.022.3432353
GTGTAGG14000.022.2898731
Produced by FastQC (version 0.11.5)