FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717637
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11950.048.423291
TCTGTCG12050.048.3937538
CTGTCGC15450.038.675949
TCGCTCT13450.038.0024344
TCTCGCT14850.036.359522
CTCGCTC15050.033.0056233
TAATACG2051.2212695E-831.6055974
GTCTTGC24350.031.2845211
CGCTCTG14600.031.0644045
GTATAAC4800.028.9939581
ACCGTGT7750.028.7972728
GTACTAC4650.028.3540231
GTGTAAC8400.026.159961
GTACGGG2009.847407E-625.6367631
GTATAGT6750.024.958541
GTCTCAC29450.024.623231
TATTCCG2051.3638528E-524.582135
GTATTAC5100.024.4159621
GTATTAG9100.024.1476551
GTTACAC7200.023.9968413
Produced by FastQC (version 0.11.5)