FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642438
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.038.8800968
CTGTCGC13350.031.8203059
GTCTTGC25550.031.8018421
GTCTCGC11700.031.00341
GTATAGG6000.027.8100511
GTGTAGC11400.027.364581
GTATAAC4600.026.8112011
TCGCTCT11550.026.8042474
TTGCTCT32200.026.6077564
CTCGCTC12450.026.0231763
GTGTAAC7550.024.98341
TCTCGCT13450.024.6236652
GTGTTAG18200.023.916911
GTGTAAT10100.023.7037871
GTCTCAC31250.023.6796531
GTGTAAG12600.023.6068741
CGTTACC2151.9704754E-523.4410322
GTATAAA9850.022.8323521
GTACGGA2252.6401422E-522.5704751
GTATTAG9750.022.3224491
Produced by FastQC (version 0.11.5)