FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642438
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.037.619191
TCTGTCG9750.034.7028928
TCGCTCT12250.031.146714
GTCTTGC22850.030.7781541
CCGTTCG1703.1846012E-629.6429589
GTATTAG8200.028.5886521
CTCGCTC13300.028.146483
ATCCGCG1555.774942E-527.8671117
TCTCGCT13500.027.196752
GTATTAA7600.026.9899441
CTGTCGC12700.026.6419819
GTGTTAG18250.026.4934581
CGCTCTG14000.025.7107285
GTGTTAC9800.025.4161851
TAGCGCT2001.1263299E-525.1965144
TCCGTAC2001.1263299E-525.1965143
GTTAGCC14550.024.2440683
GTCTCAC30700.024.1013031
GTCTTAC9300.023.6317481
GTACGGC1851.9215287E-423.348123
Produced by FastQC (version 0.11.5)