FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524604
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9050.037.390958
GTCTTGC22950.031.6314431
CTGTCGC11250.030.718929
GTCTCGC9150.028.5616361
TCGCTCT10800.025.331944
CTCGCTC11050.024.758823
GTGTTAC12100.023.998071
GTACACG2802.3163557E-723.3338381
GTATTGC6650.022.924471
GTGCGAA2252.6271264E-522.584851
GTCCTAC7750.022.4807741
TTGCTCT33550.022.1031074
GTATTAG8550.022.0754181
CGTTAAG1650.00270947221.816982
GCGTAGC2003.0777202E-421.7782481
GTATTAA7800.021.405971
ACCGTGT7400.021.4046548
GGTCTAC5800.021.2775971
GTGTAAG13000.021.2198331
GTGTAAA15450.021.143931
Produced by FastQC (version 0.11.5)