FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524604
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.035.8111428
GTCTCGC9700.030.2262151
CTGTCGC12300.029.8485589
GTCTTGC23750.029.0108051
TCGCTCT11150.028.40774
GTATTAA8150.026.981071
GTCTAAT7400.025.7535531
GTCTTAC10000.025.6545031
TCTCGCT10950.025.6399612
GTACTAC4505.456968E-1222.8041
GTATAAA11350.022.6030851
CGCTCTG12800.022.4961575
GTACTAT6200.022.4624651
TAATACT7800.022.1500634
GTACGGT1650.00271129321.8144553
GGGTAGT7750.021.7531261
GTATTAT7800.021.6136841
CTCGCTC13350.021.5693473
GTGTACG3405.736547E-821.5584031
GTATTAG9650.021.267981
Produced by FastQC (version 0.11.5)