FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520077
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8250.033.162848
GTCTCGC10300.033.110461
GTATCGG1551.4565394E-632.7695851
GTCGAAC1000.006285850428.7993098
GTCTTGC20800.028.6058651
CTGTCGC10050.027.9396279
TCGCTCT11100.027.241514
GTATTGA7550.026.9101221
GTGTTAA11400.025.4600771
TCTCGCT12250.025.271862
GTATAAT8100.025.0828931
GGTATAC3801.2732926E-1124.8235761
GCGTAGT1801.5124909E-424.1870731
GTGTATA13250.024.0958021
CTACGCT2752.1085907E-723.5621364
CTCGCTC12300.023.4131433
TACGCTT1851.920522E-423.3498655
GTGTTAC9950.023.3362711
GTATTAG8100.023.2912581
GGAGTGC54450.023.2712465
Produced by FastQC (version 0.11.5)