FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520077
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10100.037.0638668
CTGTCGC13000.031.5645989
GTCTCGC9550.030.694661
GTCTTGC20950.030.0829641
TCGCTCT11850.024.9082324
GTCCTAC7650.024.906811
GTCTCAC29200.024.5951481
GTATAGA6750.023.8849951
GTATTAT8000.023.8171391
GTCTTAC8050.023.6692071
GTGTAAG12200.023.4266931
GTACTAG5400.023.0707321
TAATACT7400.022.375544
CTCGATC10300.022.365658
CGCTCTG13650.022.1510355
TCTCGCT13150.021.8987982
GTATAAT7700.021.8898771
GTGTTAG16550.021.6972311
GTTTCAC35900.021.657423
TTAGGCG2003.2563152E-421.597263
Produced by FastQC (version 0.11.5)