FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720410
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.046.1886378
CTGTCGC13650.037.4505129
TCGCTCT12350.036.1450544
GTCTCGC12000.035.0889131
GTCTTGC25500.034.4483451
GTATTAT8850.027.0703491
TACACCG2405.646143E-826.9994775
GTCTAAT7150.026.3991851
CGCTCTG14750.026.3588125
TCTCGCT14250.026.2731782
TAGCGGT1959.246554E-625.8456554
GTCTTAC11250.025.1672211
GTACCGA1751.2450556E-424.890661
CTCGCTC15200.024.6311053
TTGCTCT34600.023.7220864
GTATAGA7700.023.5707021
GTGTTAC10550.023.3964331
GTATAAC5000.023.2312831
GTGTAAC8250.022.8792921
GTATTAG7750.022.4818861
Produced by FastQC (version 0.11.5)