FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720410
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10800.036.6469351
TCTGTCG11200.036.6376848
GTCTTGC22750.036.0831341
TCGCTCT11300.033.1280674
GTATAAG6900.030.804671
GTATTAG8950.029.481331
TCTCGCT13200.027.8147642
GTGTTAC9550.027.62911
CTCGCTC13550.026.5645163
CTGTCGC14750.026.3551169
GCGTTAT2009.799585E-625.6528551
GTCTTAC10700.025.3446061
GTGTAAC8100.025.3361511
TAATACT7450.025.1239724
ACCGTGT7550.024.7912038
GTATATA10100.024.6731831
CGCTCTG13150.024.6353035
GTGTAAT10750.024.5449241
GTGTTAG15900.023.5093561
GTACTAC4750.023.1454321
Produced by FastQC (version 0.11.5)