FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613884
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.041.1413238
CTGTCGC14450.032.8845869
GTCTTGC23300.030.837121
GTCTCGC10800.028.8961011
TCGCTCT11500.028.172864
GTGTAAC6350.026.2874641
GTATAGG7750.024.3481641
GCGCTAG3001.6907507E-824.1920851
GTGTAGA14000.023.8464851
GTATAGC5600.023.3280831
TTGCTCT32100.023.1019444
GGTCCGA2202.2125218E-523.0924451
GTATTAG8600.022.7855681
TCTCGCT13600.022.7638572
GTATTAT8400.022.4640791
GGTATAC4950.021.9928051
CTCGCTC13750.021.468293
GTATTAA7100.021.4662151
TTACACT12100.021.4206894
GTGTATA12550.021.3969841
Produced by FastQC (version 0.11.5)