FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613884
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11550.042.3843888
GTCTCGC11150.040.7345621
CTGTCGC13950.037.1567089
GTCTTGC24750.032.558451
TCGCTCT13500.031.4627864
CGTTTCG1504.617949E-528.796459
TATTCCG2054.3002547E-728.0940995
CTCGCTC15800.026.8827613
TCTCGCT15600.026.7664432
GTATTAA8150.026.0667361
GTGTAGT11950.025.7470591
CGCTCTG14950.025.5219365
GTACTAT6100.025.2194541
GTCTCAC29200.024.8369671
GTGTAAC8100.024.4188351
GCGTAGG3051.825174E-824.0185261
TGTACCG1801.5956431E-423.9970425
GTGTAAT10650.022.6992021
GTGTATA13150.022.283351
TCACTCT35000.022.2144034
Produced by FastQC (version 0.11.5)