FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536128
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14050.036.8948448
TCGCTCT17050.033.3622444
GTCTCGC15850.033.1656951
GTGTAAC10450.031.6983761
GTCTTGC30950.030.9447151
CGCTCTG19100.027.893815
CTCGCTC19650.027.8486123
TCTCGCT19900.026.4132772
CTGTCGC20800.026.3063019
GTCTTAC12800.025.8787561
GTGTTAG23850.025.6640991
GTGTAAA21850.025.0471121
GTATTAA9950.024.6065861
GTATTAG11650.023.4883861
GTATAGA10450.023.4292351
TTGCTCT44850.022.7970354
GTCCTAT10000.021.6031321
GTGTAAG17300.021.2285121
GGAGTGC78050.021.1238045
GGTATAC6500.021.0492081
Produced by FastQC (version 0.11.5)