FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536128
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16200.035.118778
GTCTCGC14900.031.8946131
TCGCTCT14800.031.1344
CTGTCGC22300.028.0958129
GTATTAA10550.027.3002831
GTCTTGC29850.026.2931021
GTGTAAA19850.025.7547591
CGCTCTG17350.025.7281685
GTATTAC8950.024.940111
GTATTAG12000.024.601541
CTCGCTC17300.024.1378483
TCTCGCT18600.023.9991262
GTACTAT8100.022.2236121
GTATATA14400.022.0013751
GGAGTGC75950.021.8978215
GTGTTAC13500.021.8680321
GTCTCAC38600.021.82521
GTACGGA2654.016272E-621.7372071
GGGTTTC58150.021.6694551
GTATAGA11750.021.448151
Produced by FastQC (version 0.11.5)