FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669123
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14550.038.100538
GTCTCGC13750.034.043351
TCGCTCT16350.028.6254394
GTCTTGC33350.027.8557951
CTGTCGC20600.025.5128489
TCTCGCT17850.025.0104712
CTCGCTC18400.024.6542113
GGAGTGC79550.024.254815
GTATAAC6850.024.1801471
GTGTAAA19450.023.6963831
CGCTCTG19800.023.6347735
GAGTGCA87100.022.8962766
GTATTAT11050.022.8101121
GTCTTAC13650.022.6859761
TTCACCG9250.022.5714555
GTGTTAG23750.022.4382921
TAATACT10050.022.2101864
GTATTAG12750.022.0280511
GTCTCAC40800.021.7103391
CTGGAGT96750.021.5831783
Produced by FastQC (version 0.11.5)