FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669123
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17000.038.9747058
GTCTCGC16650.035.0288541
GTCTTGC34000.033.884121
TCGCTCT19200.032.6231464
CGCTCTG20250.031.287115
CTGTCGC21300.030.7684579
GTCTTAC13700.028.9066181
CTCGCTC21550.026.727023
TATACTG9850.026.3132235
GTCTCAC38050.026.114371
TCTCGCT21800.025.7610572
GTATTAC9050.025.4598921
TTGCTCT44550.024.4026534
GTACTAG7200.023.00121
GTGTAAA20250.022.7567421
GTGTTAG22400.022.5011731
GTGTAAG17800.022.2483521
GTATTAA10050.022.2101141
GTATAAA14400.022.0011481
GTGTAAT15050.021.0509321
Produced by FastQC (version 0.11.5)