FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3514895
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15250.036.8244328
GTCTCGC14800.033.0841751
GTCTTGC33100.029.5858461
TCGCTCT17150.028.1289084
CTGTCGC20600.027.9597999
GTGTTAC15000.027.8426021
TCTCGCT18700.025.4123332
GTATAAT9650.025.3702471
GTATTAG11350.024.1079791
TTACACT16800.023.5719554
CGCTCTG19700.023.389725
GTGTTAG22800.023.3706051
GTATAGA10250.023.1826551
GTATTAC9050.023.0739781
GTATTGT11650.022.2510071
GTCTCAC40150.021.8800031
GTCCTAC11200.021.8591861
TTGCTCT43150.021.5253934
GTGTAAA19800.020.729211
GTGTAAC9750.020.6788561
Produced by FastQC (version 0.11.5)