FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3514895
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16550.035.6760331
TCGCTCT16700.035.352074
TCTGTCG13950.032.521028
GTCTTGC29750.030.9801621
CTCGCTC18600.030.966673
TCTCGCT20250.028.4438632
GTATTAG11450.028.298781
GTCTTAC13550.028.1641881
CGCTCTG18800.027.9564785
GTACTAG5850.027.0786821
GTATTAA9450.025.9064451
GGATACG3109.313226E-1025.5500471
GTATTAC9300.024.7758031
GCGTATA2101.6413655E-524.0015581
CTGTCGC19900.023.8829869
GTGTACG5500.023.5651661
GTCTAGC14750.023.432031
TAACACT16700.023.280634
GGTATAC7300.022.6864051
GGAGTGC78100.022.21695
Produced by FastQC (version 0.11.5)